Experiment 1: Enzyme Function

Enzyme Activity of Peroxidase Pre-lab Questions

Before attending the first lab please read the section in your textbook (and/or other resources) about how enzymes function and post answers to the following questions to your personal lab website (accessible at http://biology.westfield.ma.edu/Biol129Labs/). Also, please read the Peroxidase Lab Handout and post null and alternative hypotheses for Experiments 1-4 on your personal lab website. To access your personal lab website you must login using your WSU username and password. After you login, select your lab section from the menu on the left which will take you to a page with your name at the bottom. Select your name to access your personal lab webpage.

YOU MUST POST YOUR RESPONSES TO THE QUESTIONS BELOW AND YOUR HYPOTHESES AT LEAST 24 HOURS PRIOR TO THE START OF YOUR LAB TO EARN CREDIT.

  1. What is meant by "rate of reaction"?
  2. What effect does raising the temperature of a chemical reaction have on the rate of that reaction? Also, please sketch on a piece of paper this expected relationship between temperature and rate of reaction, and make certain to label the axes of your graph. Capture an image of that sketch with your smart phone/camera and attach it to your personal lab website.
  3. How does the concentration of reactants in a chemical reaction change over time? Also, please sketch on a piece of paper this expected relationship and make certain to label the axes of your graph. Capture an image of that sketch with your smart phone/camera and attach it to your personal lab website.
  4. How does the concentration of products in a chemical reaction change over time? Also, please sketch on a piece of paper this expected relationship and make certain to label the axes of your graph. Capture an image of that sketch with your smart phone/camera and attach it to your personal lab website.
  5. How does the concentration of enzyme in a catalyzed chemical reaction affect the rate of that reaction? Also, please sketch on a piece of paper this expected relationship and make certain to label the axes of your graph. Capture an image of that sketch with your smart phone/camera and attach it to your personal lab website.
  6. How does the concentration of substrate in a catalyzed chemical reaction affect the rate of reaction? Also, please sketch on a piece of paper this expected relationship and make certain to label the axes of your graph. Capture an image of that sketch with your smart phone/camera and attach it to your personal lab website.

Go to the Enzyme Activity of Peroxidase website .